Yoghurt production using pinecone and investigation of the effect of the produced yogurt on ECV304 cell line

OBJECTIVE: The goal of this study was to ferment yoghurt from milk using lactic acid bacteria found in pinecones and examine the cell viability lipid peroxidation levels fermented on Vascular Endothelial Cell Line ECV304.
 METHODS: ECV304 cultured vitro. To determine viability, various concentration yogurt extract with pinecone were given cells MTT Assay. At same doses as Assay, Lipid Peroxidation (LPO) Assay employed evaluate malondialdehyde (MDA) cells. Acridine orange/Ethidium Bromide staining technique applied detect apoptosis. Gas Chromatography-Mass Spectrometry used identify volatiles pinecone. RESULTS: All dosages enhanced human healthy vascular endothelial line decreased MDA levels, validated by fluorescence microscopy pictures. primary essential oils identified hexadecanoic acid, eicosanoic stearic 2-palmitoylglycerol. CONCLUSIONS: In work, effects lines examined for first time. discovered that promotes proliferation while decreasing oxidative stress these